THE FLUOROMETRIC DETERMINATION OF MALIC ACID
نویسندگان
چکیده
منابع مشابه
The fluorometric determination of malic acid.
A sensitive and specific chemical method for the estimation of malic acid in biological materials has long been desired. The condensation of malic acid with orcinol in the presence of concentrated sulfuric acid to form the highly fluorescent homoumbelliferone (7-hydroxyd-methylcoumarin) was reported years ago (1). Although this reaction is relatively specific for malic acid, the quantitative pr...
متن کاملPreparation of tissue extracts for the determination of malic acid.
Hummel’s (1) fluorometric determination of malic acid requires the precipitation of calcium malate by alcohol as a means of fractionation from biological materials. Fructose-l, 6-diphosphate, glucose-6-phosphate, or glycogen if present must be removed. Below is described a method for preparing extracts of tissues which applies the principles of chromatography on silica gel. Osazone formation, p...
متن کاملFluorometric determination of histidine.
A. FLUOROMETRIC METHOD for the determination of histidine was developed in response to the need for an accurate and precise manual method for the confirmation of histidinemia (1). it is based on the fluorometric method of Shore et a!. (2), which involves the condensation of histaniine with o-phthalaidehyde (OPT) in a strongly alkaline solution to form a rather labile fluorescent compound which ...
متن کاملFluorometric determination of inulin using 5-quinolineboronic acid and inulinase.
Inulin is a polysaccharide composed mainly of D-fructose units and is the most reliable indicator of the glomerular filtration rate. We have proposed an inulin detection method that involves the hydrolysis of inulin to D-fructose using inulinase and the selective binding of D-fructose from inulin using 5-quinolineboronic acid. In this method, the fluorescence of 5-quinolineboronic acid increase...
متن کاملFluorometric determination of propoxyphene.
With 4-chloro-7-nitrobenzo-2, 1,3-oxadiazole (I), fluorescent derivatives of propoxyphene can be prepared, thus enabling quantitation of the drug in biological samples. This technique offers an acceptable alternative to gas chromatographic (1-5) or ultraviolet spectrophotometric (6, 7) procedures. Favorable aspects of the fluorometric technique are high sensitivity, specificity, potential for r...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1949
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)51235-2